Background: Adults with spinal cord injuries and disorders (SCI/D) require chronic indwelling catheterization which is associated with an increased risk of catheter-associated (CA) adverse events.Methods: We studied urine samples (culture and urinalysis) from two cohorts of chronically catheterized males with SCI/D. Cohort 1 included 28 participants; three samples per patient were collected (before, after, and seven days after catheter change). Cohort 2 included 21 participants; seven samples per patient were collected (before, immediately after, 30 minutes, 1 hour, 1 day, 2 days, and 7 days after catheter change).